The motif of shifting identity that runs throughout Nimona can be read as a symbolic representation of queerness, specifically the construction of fluid LGBTQ+ identities against a normative sociocultural backdrop. Most of the characters in the story can be interpreted as queer, and they each go through shifts that offer insight into the construction of their identities and relationships.

Nimona, who can shapeshift into animals and other people, embodies a literal type of fluidity. Although she passes from one state to another effortlessly, her constant shifting underlies the instability of her identity. This becomes evident as she gradually loses control not only of her ability to transform, but also of her self-perception. At first, Nimona projects confidence through humor and emphatic mannerisms. As fragments of her past are revealed through unreliable memories and hypothetical stories, her carefully crafted facade gives way to a much more ambiguous identity. At one point, Blackheart even remarks to Nimona in her human form: “You forgot your own backstory, Nimona. It wasn’t true. I took it for granted that this was your natural form. But it’s not, is it?” (168). This highlights Nimona’s own confusion in her identity as well as the power of perception in creating false or partial identities, which are significant queer motifs.